RESUMO
Obesity is currently considered an inflammatory condition associated with autoimmune diseases, suggesting a common origin. Among other factors, candidate genes may explain the development of this disease. Polymorphisms in the tumor necrosis factor α (TNFα) and lymphoid protein tyrosine phosphatase (PTPN22) genes lead to an increased risk to development of immune and inflammatory diseases. The aim of the present study was to analyze the biochemical parameters and the effect of the TNFα -308G/A and PTPN22 +1858C/T polymorphisms in the susceptibility of adolescents to obesity. A group of 253 adolescent subjects were recruited and classified as obese, overweight or normal weight according to their nutritional status. Anthropometric measurements, clinical and biochemical data were analyzed. DNA was extracted from peripheral blood samples by the phenol-chloroform method, and TNFα -308G/A and PTPN22 1858C/T polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism assays. Clinical, genetic and biochemical parameters were analyzed to determine the existence of a possible association with the development of obesity. Statistically significant differences in body mass index, insulin, triglyceride levels and homeostatic model assessment for insulin resistance (HOMA-IR) index were observed among the three groups analyzed (P≤0.05). The studied polymorphisms did not confer a risk for developing obesity in the analyzed population (P>0.05); however, significantly low levels of insulin and decreased rates of HOMA-IR were observed in the 1858 CT genotype carriers of the PTPN22 gene. In conclusion, no association between the TNFα -308G/A and PTPN22 +1858C/T polymorphisms and the risk to development of obesity in the adolescent population analyzed was observed. However, the 1858 CT genotype of the PTPN22 gene was associated with variations of certain biochemical parameters analyzed.
RESUMO
OBJECTIVE: To identify microalbuminuria in patients with type 2 diabetes. MATERIAL AND METHODS: A descriptive cross-section study was made in patients with type 2 diabetes with more than a year of evolution. Microalbuminuria was defined as the presence of 30 to 300 mg of albumin in urine of 24 hours. The socio-demographic characteristics, type of diet, exercise, type of antihypertensive and oral antihyperglycemic agents were analyzed. Basal glycemia after one and two months, and renal state were assessed. RESULTS: Of 301 type 2 diabetic patients, 251 had microalbuminuria. The mean age was 57.2 years old. Women predominated over men (1.4:1). The mean for diabetes evolution was nine years. The complication with more frequency was hypertensive cardiopathy 78.1 %. A medium protein diet was present in 49%. 62.2% exercised and 40.2% exercised less than 30 minutes. Mean GFR with creatinine clearance was 83.3 +/- 32 mL/min. 98% of the cases received oral antihyperglycemic agents. Basal glycemia, after one and two months, was, on average, 171.8 mg/dL, 190.1 mg/dL, and 217.4 mg/dL. 74.5% of the patients had hyperglycemia in the first measuring, 79.3% in the second one, and 70.5% in the third one. CONCLUSIONS: The prevalence of microalbuminuria was greater than the reported in literature (85.3%). A poor control of glycemia was showed.
